Quantification of HDL2 and HDL3 cholesterol by the Vertical Auto Profile-II (VAP-II) methodology

Quantification of cholesterol in all lipoprotein classes by the VAP-II method.

We have developed a high resolution microvolume Vertical Auto Profile (VAP) method for the simultaneous measurement of cholesterol in all lipoprotein classes, including lipoprotein[a] (Lp[a]) and intermediate density lipoprotein (IDL). This method, designated as VAP-II, uses a non-segmented continuous flow (controlled-dispersion flow) analyzer for the enzymatic analysis of cholesterol in lipopr...

isolation of hdl2 and hdl3 assessment of concentration of cholesterol

the major lipoprotein classes are heterogenous. human serum high-density-lipoproteins (hdl) are separated into two major subfractions (hdl2, d=1.063-1.125 g/ml, hdl3, d=1.125-1.210 g/ml) by a simple double precipitation method. there was a strong inverse relation between hdl-cholesterol (hdl-c) and subfractions hdl2-c and hdl3-c with atherosclerosis. this study demonstrated that hdl-c (p<0.001)...

Decreased HDL2 and HDL3 cholesterol, Apo A-I and Apo A-II, and increased risk of myocardial infarction.

BACKGROUND A large and consistent body of evidence supports the judgment that elevation of total plasma blood cholesterol is a cause of myocardial infarction (MI) and that high levels of low density lipoprotein (LDL) cholesterol have a positive relation and high levels of high density lipoprotein (HDL) cholesterol an inverse relation with MI. At present, however, the roles, if any, of the major...

Beyond HDL-cholesterol increase: phospholipid enrichment and shift from HDL3 to HDL2 in alcohol consumers.

The reduction of cardiovascular mortality associated with moderate alcohol consumption is chiefly thought to be mediated by an increase of high density lipoprotein cholesterol (HDL-CH). This study highlights additional qualitative changes of HDL that might augment this antiatherogenic effect. In 279 healthy men, alcohol and nutrient consumption were evaluated. Groups 1 (n=62), 2 (n=172), and 3 ...

(اندازه گیری فسفولیپیدهای HDL3 ، HDL2 HDLt)